![]() If he does score some baskets, you’ll just need to score an extra shot to make up for each one. Always have him pass to you or set you up for alley oops. For the points to count, you must personally score the baskets, not your partner. To get 100 points, you really need the quarter length set to five minutes. Century scorer – Score 100 Points in 1 Game. If it makes it through the hoop and causes you to win, you’re all set! Also, you can still get a buzzer beater if the game goes into overtime. Once the last 15-30 seconds of the final quarter nears, hold the ball and avoid the enemy until the timer reaches one second, then throw the ball. To keep your AI partner from scoring points, have him pass you the ball (blue button) whenever he gets it. Every time they score a goal, you need to score your own, and vice versa. The key to setting up a buzzer beater is keeping your score tied or only one point below the opponent's score throughout the entire match. Picking a character with high 2PT and 3PT stats will help too. But this is Achievement takes eight minutes to attempt (four quarters at two minutes each) and is easy to fail, so you might want to hold off until your second Campaign playthrough (when you’ll have mastered the ropes of the game) before you try for it. The ideal time to go for ‘Buzzer beater’ is during the very first Classic Campaign game because the difficulty rises incrementally in subsequent games. Not only that, but the shot must actually cause you to win the game if your score was high enough to win before throwing the ball, it won’t count. A ‘buzzer beater’ is a thrown shot (not a dunk) that goes through the hoop at the end of the fourth quarter after the clock has already hit zero. This is probably the hardest Achievement in the game, but it’s not that bad. ![]() Unlocks: Beastie Boys team, George Gervin, Mark Price Buzzer beater - Hit a Buzzer beater shot to win the game. A character with high DNK, STL, 2PT, and 3PT stats such as the Boston Celtics’ Kevin Garnett will make several Achievements easier. Most of the single-game Achievements are easier to get with Quarter Time set to five minutes, but you can get all but ‘Century Scorer’ with two minute quarters.īecause you’ll have to play a whopping 222 Classic Campaign games to get all of the Classic Campaign Achievements, I recommend going for the single-game Achievements in Classic Campaign mode, not Play Now. For all of the Achievements in this guide, you’ll want the difficulty set to Rookie and Controls to Arcade. These eight Achievements can be earned during single games in either Play Now or Classic Campaign modes. ‘Southwest Division represent’ Achievement: Unlocks Stickman team (Red, Blue, Green, Yellow).‘100 Club’ Achievement’: Unlocks the EA Mobile team (Ryan Winterholler, Josh Valensi, Sean Chapel).‘Buzzer Beater’ Achievement: Unlocks the Beastie Boys (Mike D, MCA, and Ad-Rock).Three teams can only be unlocked by earning specific Achievements:
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![]() ![]() ![]() Cash per pop multipliers, such as Rubber to Gold, Monkey Town, or even Benjamin's own Syphon Funding, do not affect Trojan.įor example, a Rainbow bloon on round 42 targeted by a level 7 Benjamin will give m = (1)(8) + 1 dollars, or 9 dollars. In addition, Bloon Trojan's income can only be boosted by Monkey City. Trojaned bloons give 1 extra dollar from rounds 1-60. (Despite having fewer layers than a BFB, DDT bloons are considered to have 12 layers by the bloon trojan.) l = the number of layers a bloon has upon being affected by the bloon trojan.t = 1 for levels 7-15 and t = 2 for levels 16-20. t = the level of the Bloon Trojan ability.m = the amount of money given by the bloon.The amount of money a trojaned bloon gives can be calculated using the formula m = (t ⋅ l) + r where: Benjamin cannot target Camo Bloons with the Bloon Trojan ability without being given camo detection until he reaches level 18. The Bloon Trojan will not target any bloon weaker than a Pink Bloon. His upgrades can also be immediately bought with cash, though the amount of cash required to purchase the next upgrade will typically increase as the upgrades get higher, making self-upgrading an important element for his overall power.īenjamin requires 1.5x as much experience points to upgrade towards each level compared to Quincy, Gwendolin, Striker Jones, or Obyn Greenfoot.ĭescription: Syphon Funding lasts 20 seconds and cash per pop is triple normal for affected Bloons.Įffect: Syphon Funding lasts for 20s instead of 10s, and affected bloons give 3x cash.Īssuming Ben is placed in round 3 and not upgraded manually:Īs of Version 11.0, the Bloon Trojan ability gives some money back from trojaned bloons, based on the number of layers destroyed by the ability. ![]() He earns more XP each round to unlock his next upgrade irrespective of how many bloons he pops. If Hero Favors is enabled, he will cost $920 on Easy, $1,080 on Medium, $1,165 on Hard, and $1,295 on Impoppable.īenjamin will start upgrading himself every round upon placement. A Sushi Bento skin was added in version 24.0, replacing Benjamin's laptop and desk with a sushi mat. He wears slacky white clothes and a white cap which he wears backwards, and his computer is replaced with a DJ turntable and a laptop. In version 11.0, he was given a new skin called DJ Benjammin', or just Benjammin', who has new voice lines and graphics. Benjamin levels 1.5x slower than the base four heroes ( Quincy, Gwendolin, Striker, and Obyn), or 0.66x as fast. At Level 10, he gains an ability that downgrades all incoming bloons (excludes DDTs and BADs) by one rank, as well as doubling cash per pop for all affected bloons, which is increased to triple at Level 20. At Level 7, he gains a Bloon Trojan attack, which causes affected bloons to spawn no children when destroyed. Instead, he uses his hacking skills to primarily provide extra money, as well as lives starting at Level 6. Unlike other heroes, Benjamin does not attack bloons directly. He is unlocked with a one-off purchase of 3,000. Benjamin was released in Version 3.0 on August 22nd, 2018, as well as the advanced map, High Finance. Using his elite hacking skills, Benjamin can create extra money for the cause.īenjamin is a Hero in Bloons TD 6. ![]() Plus, it has the added distinction of having registered official licensing for the UFC franchise with all the biggest names and real images of the UFC fighters themselves. Its touchscreen controllers are amazing and it comes with spectacular graphics. The recommended steps are to save the license key (which youll get when. UFC Mobile 2 is an excellent fighting game. Raspberry Pi 1/Zero version Raspberry Pi 2 version Raspberry Pi 3 version You. In order to get new fighters, you'll have to open up your fair share of trading card packs just like in FIFA. Online multiplayer on console requires Xbox Game Pass Ultimate or Xbox Live Gold (subscription sold separately). Plus, in the story mode, you'll get to try your hand against a bunch of different fighters in every weight class in the UFC. EA SPORTS UFC® 2 Electronic Arts Sports TEEN Lyrics, Blood, Violence, Mild Suggestive Themes In-Game Purchases (Includes Random Items), Users Interact +Offers in-app purchases. You'll beat them one by one, until facing the final fight for the championship title. Throughout the story mode, you'll face off against different categories of fighters. EA Sports UFC 2 CD Key Generator Features Ufc 2 Pc Keygen Windows 7 First download CD Key Generator and Open it. Starting out, you'll begin playing as Brad Tavares in the middleweight class. Download EA Sports UFC 2 cd key generator for free and play in Cracked EA Sports UFC 2 PC Xbox One PS4. Once they're charged up, all you have to do is tap the button in the lower left corner to continue. Swiping your finger from up to down, or left to right will have you hitting with a hook or uppercut (they're key in recharging your special attack skills). Serial numbers for EA Sports UFC 3 PC Version License Key.txt: EA Sports UFC 3 PC Version License Key.txt serial number. EA Sports UFC 2 License Serial CdKey Registration Key Generator supports wide range of platforms, such as Windows and Mac OS X. Here is the only place where you can download EA Sports UFC 2 Serial Key Number for free. EA Sports UFC 2 License Serial CdKey Registration Key Generator file is 100 clean and safe, no hidden ads or offers, we use only open source technologies, full code is available for you to edit or upate. The game relies on cutting-edge graphics that bring a realism never. You'll need to charge up these special attack moves from time to time, though. EA Sports UFC 2 Serial Key Generator PC Xbox One PS4 EA Sports UFC 2 Serial Key Generator PC Xbox One PS4.After that open EA Sports UFC 2 Serial Key Generator and click Generate button to get your EA Sports UFC 2 Key. EA Sports UFC 2 PC Crack is the new game based on the most popular MMA event in the world. ![]() Tapping on any spot has you launching a jab that you can spin into a special attack to pack on more damage to your opponent. And of course, it's here with a whole roster of real-life players including some of the biggest names in UFC, including Adesanya, Cormier, Nunes and Khabib.Ĭontrol systems in UFC Mobile 2 are perfectly adapted to smartphone screens. This time it's back with all the excitement of one-to-one combat in the octagon. ![]() UFC Mobile 2 is the official UFC game for Android. Contact Us Art of Gifting Choose the perfect gift from our specially curated selection of products. Sarah, Louis Vuitton's legendary wallet. 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And if you have multiple Gmail accounts, you can manage them all in the same application, without having to sign in and out of your different accounts. ![]() Mailplane is a small shareware application that gives you all the features of Gmail's web interface via a desktop application. Compare price, features, and reviews of the software side-by-side to make the best choice for your business. And even though Gmail works well with desktop clients, it's just not the same experience as the web-based version.īut a few years ago I came across a shareware program called Mailplane that changed everything for me. ProtonMail and Mailplane can be primarily classified as 'Email Services' tools. Gmail client built for OSX with Calendar and Gmail support to allow usage in the familiar Gmail environment. (Does anyone remember that you used to need an "invitation" from a Gmail user just to get your own account?) These days I have several Gmail accounts (actually, some of them are Google Apps accounts-Gmail but with your own domain), some for business, some for personal, and some for junk mail. Since Ive gone back to Gmail, having all of these abilities at my fingertips without having to resort to using Gmail on the web (or Mailplane, for that. What is Mailplane An e-mail client for Mac OS X that 'wraps' the Gmail service as a site-specific browser. I've been using Gmail for what seems like ages. ![]() Tons of features, a generous storage amount, it works with desktop clients like Apple Mail or Thunderbird, and it's free. I think Gmail is the best email service there is. A brief review of Mailplane from a longtime user. ![]() Special phone keypad characterĭial by Name - Name recognition with speech Pressing the star key (*) will repeat the list of matching names to the person. For example, the person will be asked to use the pound key (#), while the zero (0) key is used for a space between names. There are several special characters that are used when searching for people using a phone keypad. Press 1 for Mary Marble Press 2 for Mary Jones Press 3 for Amos Marcus Press 1 for Amos Marble Press 2 for Mary Marble Press 1 for Amos Marble Press 2 for Amos Marcus ![]() People can search for names in your organization using the following search formats on their phone keypad: Name format If more than 5 names remain after any Dial Scope Include or Exclude lists have been applied, the search will fail and the caller will be told that too many names were found. For example, "After you enter the name of the person you are trying to reach, press #." If there are multiple names that are found, the person calling will be given a list of names to select from. When they are entering the name, they will be asked to terminate their keypad entry with the # key. When searching your organization's directory, people can use the '0' (zero) key to indicate a space between the first name and last or last name and first. There are various formats that can be used when the name is entered. ![]() People calling in can use Dial by Name to reach users by specifying either the full or partial name of the person they are trying to reach. If the initial search against the entire directory returns more than 100 users, the Dial Scope lists will not be applied, the search will fail, and the caller will be told that too many names were found. Search Considerationsĭial by Name searches the entire organization's directory first before applying any Dial Scope Include or Exclude lists that have been configured. You can use the Dial Scope feature to narrow down the names that are reachable by changing the scope of Dial by Name for a particular auto attendant. If you are using Dial by Name with speech recognition, but your organization's Active Directory is larger than 80,000 users and you haven't limited the scope of Dial by Name using Dial Scope feature, Dial by Name will still work for your callers using a phone keypad, and voice inputs will be available for all other scenarios. Partial FirstName + LastName LastName + FirstNameįirstName LastName FirstName + LastName LastName + FirstName Maximum number of users in an organization The maximum name list size that a single auto attendant can support using speech recognition is 80,000 users. A caller can enter partial or full names (FirstName + LastName, and also LastName + FirstName), but needs the full extension number. There is no limit on the number of Active Directory users Dial by Name and Dial by Extension can support when a caller searches for a specific person. Given the prerequisites involved, Dial by Name or Extension must be explicitly enabled when configuring an auto attendant. For multi-national organizations, Dial by Extension will find and transfer callers to Microsoft Teams users who are in different countries or regions. You will also need to have an appropriately configured dial plan for your users. Teams users who can be reached by using Dial by Extension aren't required to have a phone number or have Calling Plans assigned to them, but they must be Enterprise Voice enabled for Skype for Business Server users. For multi-national organizations, Dial by Name will find and transfer callers to Microsoft Teams users who are in different countries or regions. Teams users who can be reached by using Dial by Name aren't required to have a phone number or have Calling Plans assigned to them, but they must be Enterprise Voice enabled for Skype for Business Server users. Dial by Name or Dial by Extension are options you set up when you configure the call flow settings in an auto attendant. The auto attendant will search the company directory, locate the person, and then transfer the caller to them. Using their voice or phone keypad callers can say or enter the full or partial name, or extension of the person they would like to reach. Dial by Name or Extension is an auto attendant feature that enables callers to reach Teams users in your organization. ![]() ![]() Firstly, the insert is PCR-amplified using primers with 5′ ends complementary to the target site in a circular destination vector. In contrast, OEC has two rounds of amplification. After mixing, these single-stranded overhangs anneal and can be efficiently ligated in vivo after transformation by the bacterial DNA recombination and repair machinery. These are analogous to the much shorter ‘sticky ends’ generated by restriction enzymes. ![]() Cloning is possible due to the complementary 5′ ends of the insert and vector fragments. SLIC uses a brief treatment of purified PCR products with the 3′→5′ exonuclease activity of T4 DNA polymerase to generate a higher proportion of recessed ends. PIPE relies on the observation that a significant portion of PCR products are incomplete, having 3′-recessed ends, particularly in the absence of a final extension step. The 3′ ends of the PCR primers are template-specific, whilst the 5′ ends incorporate tails specific for the cloning junction. (C) Colonies from ampicillin plates were patched onto kanamycin/X-gal plates to distinguish recombinants from unwanted insert vector or empty pUC18/Kan background colonies.Īll three techniques amplify the gene of interest by polymerase chain reaction (PCR). (B) Design of the reporter vector, encoding resistance for ampicillin and kanamycin, and the alpha-fragment of beta-galactosidase. For all techniques, a DpnI digestion step is included to remove plasmid template (not shown). In OEC, the insert PCR product acts a megaprimer to generate a nicked plasmid by overlap extension in vitro in a second round of amplification. In both these techniques, by amplifying vector and insert with primers containing complementary 5′-tails and mixing the products, the overhangs can anneal and are joined in vivo after transformation into E. In SLIC, purified PCR products are treated with T4 DNA polymerase (DNAP) so that the exonuclease activity will increase the proportion of recessed ends. (A) In PIPE, incomplete extension during PCR generates 3′-recessed ends. Principles of polymerase incomplete primer extension (PIPE) cloning, sequence and ligation-independent cloning (SLIC) and overlap extension cloning (OEC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist.įigure 1. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This work was supported by the National Health and Medical Research Council (1008081, and the National Heart Foundation of Australia (G11S5757, ). Received: OctoAccepted: NovemPublished: December 23, 2013Ĭopyright: © 2013 Stevenson et al. PLoS ONE 8(12):Įditor: Jean Peccoud, Virginia Tech, United States of America These ligation-independent cloning approaches constitute an essential part of the researcher's molecular-tool kit.Ĭitation: Stevenson J, Krycer JR, Phan L, Brown AJ (2013) A Practical Comparison of Ligation-Independent Cloning Techniques. Our data suggest that for small inserts (<1.5 kb), OEC is a good option, requiring only two new primers, but performs poorly for larger inserts. Overall, PIPE achieved cloning efficiencies of ∼95% with few manipulations, whereas SLIC provided a much higher number of transformants, but required additional steps. Experiments made use of a common reporter vector and a set of modular primers to clone DNA fragments of increasing size. Here, we outline and optimise these techniques and identify important factors to guide cloning project design, including avoiding PCR artefacts such as primer-dimers and vector plasmid background. These strategies rely on the generation of complementary overhangs by DNA polymerase, without requiring specific restriction sites or ligation, and achieve high efficiencies in a fraction of the time at low cost. A number of ligation-independent cloning techniques have been developed, including polymerase incomplete primer extension (PIPE) cloning, sequence and ligation-independent cloning (SLIC), and overlap extension cloning (OEC). Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and inefficient enzymatic steps. The precise assembly of specific DNA sequences is a critical technique in molecular biology. ![]() Hive support has confirmed that they will soon be adding multiple accounts feature to the platform.Ģ. However, they are working to increase it.ġ. For those unable to sign into the app or enter password while logging in, Hive support says you can try logging in using Google or Apple accounts as email verification quota is currently limited. The team is working to resolve issues with hashtags.Ĥ. Also, an issue where NSFW posts retain the NSFW status on reposts is being patched.ģ. They have confirmed that a fix is coming soon for making Gallery load easier.Ģ. We’ll keep tabs on the issue and update this article as and when we come across anything noteworthy.ġ2:10 pm (IST): Hive support is now actively responding to user complaints regarding ongoing bugs and has also announced some new features that are coming soon to the platform.ġ. grant permission for ‘photos and videos’Īnother one involves the following: Source Uninstall the app, and reinstall it but DON’T sign-inĤ. The first one involves granting permission for the app to access your photos and videos:ġ. Hive Social tweeted today that the platform will soon be receiving several improvements that will enhance user experience: Sourceįortunately, we have come across potential workarounds for the issue where some Hive Social users are unable to upload photos from the gallery. Hive Social users are demanding a web or desktop version ( 1, 2, 3, 4, 5) of the platform to match the competition. But so far, some of those who can aren’t quite impressed with the app ( 1, 2, 3, 4, 5). The Android app is currently in beta which explains why not everyone can access it. Other users are reporting that they can’t use their existing images from their phone’s gallery to set as avatars or banners ( 1, 2, 3, 4, hi i just made an account and i can’t seem to use existing images on my phone to set as my avatar or banner. Some big things from Android is some jittering on scroll, the app freezing then crashing when refreshing the feed, and dark mode seems to reset when closing the app. I’m assuming everybody saying positive things about Hive Social has an iPhone, because I’m trying it on my Android phone and the damn thing keeps crashing! Thought it was just when I was trying to set up my profile, since it let me post, but nope!įirst impressions are good. Some report that it crashes when uploading their avatar, while others experience when scrolling through posts.Įarlier, some assumed that Hive Social is crashing because their servers are unable to cope with the influx in traffic.īut the prevalence of this issue for the past 4 to 5 days suggests there could be something wrong with the Android app. The app often crashes with varied usage patterns. This makes it impossible for users to set usernames, add profile pictures and use the platform in general ( 1, 2, 3, 4, 5, 6, 7, 8, 9). The recently launched Hive Social Android app is currently experiencing severe crashing issues. However, there are some concerns regarding Hive Social that are being reported and readers should know before trying their hands on the platform. So, they regularly look for platforms that are more natural. Gen-Z doesn’t want to be monitored and manipulated by algorithms that seem to be getting more and more aggressive day by day. It’s a new app trying to be twitter but without Elon lol. Some feel that it’s just Twitter but without the influence of Elon Musk ( 1, 2, 3, 4). I’m just clicking around hoping I’m doing it right Getting used to the Hive Social app is reminding me what it was like when I first started using social media. Some of the features include profile music, profile banners, image/GIF/text posts, and much more.Ĭurrently, Hive Social is trending on Twitter where users are migrating to the platform and are impressed with the first looks of it ( 1, 2, 3, 4, 5, 6, 7, 8). Well, it is a new social media platform that is similar to Instagram in some respects but comes with a chronological feed. ![]() You must be wondering what Hive Social is. And it looks like after Mastodon, the shift is now going towards Hive Social. However, after buying the platform, things seem to have spiraled out of control as there has been mass layoffs, Twitter Blue subscription and the new whole Twitter 2.0 chaos.įor some, Twitter has been a mess lately and that’s why others have been searching for potential alternatives. He started his quest to acquire the widely popular social media platform this January, the same month he began buying Twitter shares. Original story (published on November 21, 2022) follows:Įlon Musk’s acquisition of Twitter has been tumultuous, to say the least. New updates are being added at the bottom of this story……. ![]() The animals’ diplomatic importance has led to some controversy. They argue that pandas form a key part of guanxi–reciprocal relationships that can establish deeper and more trusting bonds between countries. Kathleen Buckingham and Paul Jepson of Oxford University found recent panda loans coincided with trade deals that China had signed in Scotland, Canada and France. But the bears were not offered to just any country. Instead of giving them away, in the 1980s China started loaning them for $50,000 per month, with the bears and any offspring remaining Chinese property. ![]() But as China has grown increasingly capitalist, pandas have become an economic tool as well. By giving its national animal to a foreign power, China is able to emphasise the closeness of political ties. Russia and North Korea were given pandas during the cold war, and America got a pair after President Nixon’s China trip in 1972. The tradition resurfaced under Mao Zedong. Why does Malaysia have to pay so much?Ĭhina has offered pandas as gifts since the seventh century, when Empress Wu sent two bears to Japan. The new Malaysian government, which took power in May 2018, has been reviewing the deal, and this month the leader, Mahathir Mohamad, had to refute ministerial claims that the pandas would be returned early. ![]() As part of an agreement made in 2014 by its then prime minister, Najib Razak, Malaysia must pay China $1m each year to rent the bears until 2024. So expensive, in fact, that the Malaysian government has reportedly considered handing its two adult pandas back to China, the country from which all pandas originate. This describes the degree to which the sensing end of the thermocouple is exposed to the environment it's measuring.
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